Milky Spore Powder is a One Time application using only one teaspoonful every 4 feet in a checker board pattern on your lawn. Apply anytime the ground is not frozen. This product is not harmful to man, animals or the environment. It is safe to use around water ways. Kills the grub stage of the Japanese Beetle by the natural bacteria eating the grub from the inside. Does not harm beneficial insects. 10 oz. will treat up to 2,500 sq ft.